Investigation of Antibacterial Activity of Rhamnus Cathartica L. and Its Anti-qs Potential on Pseudomonas Aeruginosa Pao1

Strategic Insights -

Research conducted at Suleyman Demirel University in Isparta, Turkiye, has discovered that the R. cathartica L. seed extract exhibits antimicrobial activity against both Gram-positive and Gram-negative bacteria, including Pseudomonas aeruginosa. The study found that the extract's inhibitory effect on P. aeruginosa PAO1 was moderately significant, with a notable reduction in elastase, pyocyanin, and biofilm production. Computational analysis revealed that the main component of the extract, kaempferol, can bind to QS system receptors, thereby inhibiting the QS system. This study provides a new perspective on using R. cathartica L. seed extract as a green antibacterial agent against Pseudomonas aeruginosa.

Key Takeaways:

  • The R. cathartica L. seed extract exhibited antimicrobial activity against both Gram-positive and Gram-negative bacteria, with notable effects on Pseudomonas aeruginosa PAO1.
  • The inhibitory effect of R. cathartica L. extract on P. aeruginosa PAO1 was measured as follows:

+ Elastase: 61% inhibition

+ Pyocyanin: 18% inhibition

+ Biofilm: 61% inhibition

  • Computational analysis identified kaempferol as the main component of the extract responsible for its anti-QS potential.
  • Kaempferol's binding potential on QS system receptors was investigated via molecular docking, showing that it can prevent competitive ligands from connecting to LasR.
  • The study suggests that R. cathartica L. seed extract may be a viable green antibacterial agent against Pseudomonas aeruginosa.
  • Suleyman Demirel University's Faculty of Pharmacy, Department of Pharmaceutical Microbiology, is based in Isparta, Turkiye.
  • Additional researchers involved in this study include Ahu Reis, Ulas Evren Arin, Ebru Onem, and Muhammed Tilahun Muhammed.
  • Keywords for this study include Isparta, Turkiye, Eurasia, Gammaproteobacteria, Gram-Negative Aerobic Bacteria, Gram-Negative Aerobic Rods and Cocci, Gram-Negative Bacteria, Proteobacteria, Pseudomonadaceae, Pseudomonas, Pseudomonas aeruginosa.

Statistics:

  • 61% inhibition of elastase production in P. aeruginosa PAO1 by R. cathartica L. extract
  • 18% inhibition of pyocyanin production in P. aeruginosa PAO1 by R. cathartica L. extract
  • 61% inhibition of biofilm production in P. aeruginosa PAO1 by R. cathartica L. extract
  • Molecular docking analysis revealed that kaempferol binds to QS system receptors with high affinity

Sources:

  • Investigation of the Antibacterial Activity of rhamnus Cathartica L. and Its Anti-qs Potential On pseudomonas Aeruginosa Pao1. Natural Product Research, 2025;39(16):4762-4774.
  • Natural Product Research can be contacted at: Taylor & Francis Ltd, 2-4 Park Square, Milton Park, Abingdon OX14 4RN, Oxon, England.
  • Suleyman Demirel University, Faculty of Pharmacy, Dept. of Pharmaceutical Microbiology, Isparta, Turkiye.