Advances in Nanoparticles: New Method for Determining Boron Content in Tissues

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A new study published in Atomic Spectroscopy has proposed a method for determining the boron content in organs and tissues after the administration of boron nanoparticles (BNPs) to laboratory animals. The study, conducted by researchers at the Russian Academy of Sciences, aimed to optimize a method for analyzing the boron content in tissues without the need for dissolution and preliminary freeze drying of samples. Financial support for this research came from the Russian Science Foundation (RSF).

The proposed method involves ashing biological samples in a tubular quartz furnace and analyzing the resulting powders using two-jet plasma optical emission spectrometry (TJP OES). The researchers optimized the conditions for sample preparation and quantitative analysis, achieving a limit of quantification in different tissues ranging from 5 to 200 ng g-1. The accuracy of the proposed method was confirmed by spike experiments, with a boron recovery of not less than 97%. The method was developed for the analysis of various organs and tissues, including tumors, brain, skin, muscle, kidneys, liver, and spleen.

Key Takeaways:

  • A new method has been proposed for determining the boron content in organs and tissues after the administration of BNPs to laboratory animals.
  • The method involves ashing biological samples in a tubular quartz furnace and analyzing the resulting powders using TJP OES.
  • The limit of quantification in different tissues ranged from 5 to 200 ng g-1.
  • The accuracy of the proposed method was confirmed by spike experiments, with a boron recovery of not less than 97%.
  • The method was developed for the analysis of various organs and tissues, including tumors, brain, skin, muscle, kidneys, liver, and spleen.
  • The maximum boron content at each time point was determined in the spleen, with values of 0.5, 1, and 2 hours after administration being 20, 3.0, and 6.6 %ID g-1, respectively.

Statistics:

  • The limit of quantification in different tissues ranged from 5 to 200 ng g-1.
  • The accuracy of the proposed method was confirmed by spike experiments, with a boron recovery of not less than 97%.
  • The maximum boron content at each time point was determined in the spleen, with values of 0.5, 1, and 2 hours after administration being 20, 3.0, and 6.6 %ID g-1, respectively.

Sources:

  • "Two-jet Plasma Optical Emission Spectrometry Determination of Boron In Animals' Tissues and Biodistribution After Administration of Boron Nanoparticles." Atomic Spectroscopy, 2025;46(4).
  • Russian Academy of Sciences, Nikolaev Institute of Inorganic Chemistry, Siberian Branch, Acad Lavrentiev Ave, Novosibirsk 630090, Russia.
  • Atomic Spectroscopy Press Ltd, Flat H29, 1-F, Phase 2 Kwai Shing Ind Bldg, No 42-46 Tai Lin Pai Rd, Kwai Chung, Kwai Tsing, Hong Kong.