Accurate Lysozyme Detection in Human Biofluids Using AuNPs-GO/MIP

Strategic Insights -

Researchers from Bolu Abant Izzet Baysal University in Turkey have developed a plasmonic sensing platform that utilizes gold nanoparticles (AuNPs) and graphene oxide (GO) through molecular imprinting for the detection of lysozyme in human biofluids. This platform enables sensitive, low-cost, and label-free lysozyme detection in buffer and synthetic biofluids. The sensor exhibits a high repeatability and selectivity for lysozyme compared to other proteins.

Key Takeaways:

  • The AuNPs-GO/MIP sensing platform integrates gold nanoparticles and graphene oxide through molecular imprinting for the detection of lysozyme in urine, serum, and saliva.
  • The sensor enables sensitive, low-cost, and label-free lysozyme detection in buffer and synthetic biofluids with a detection limit of 0.0015 nM.
  • The sensor exhibits a linear detection response for lysozyme in buffer (pH 7.4 PBS) within 0.01-0.5 nM.
  • The sensor shows 4.27 times higher selectivity for lysozyme compared with myoglobin and 2.36 times compared with cytochrome c.
  • The sensor exhibits high repeatability (RSD = 2.14%) and a remarkable imprinting factor (IF: 4.98).

Statistics:

  • Detection limit: 0.0015 nM
  • Linear detection response: 0.01-0.5 nM
  • Selectivity: 4.27 times higher for lysozyme compared with myoglobin and 2.36 times compared with cytochrome c
  • Repeatability: RSD = 2.14%
  • Imprinting factor: IF = 4.98

Sources:

  • Lysozyme detection in body biofluids by dual-decorated graphene oxide-gold nanoparticles-based biomimetic plasmonic sensor. Microchimica Acta, 2025;192(9):606. Microchimica Acta can be contacted at: Springer Wien, Prinz-Eugen-Strasse 8-10, A-1040 Vienna, Austria. (Springer - www.springer.com; Microchimica Acta - www.springerlink.com/content/0026-3672/)
  • Researchers at Bolu Abant Izzet Baysal University Have Reported New Data on Gold Nanoparticles (Lysozyme detection in body biofluids by dual-decorated graphene oxide-gold nanoparticles-based biomimetic plasmonic sensor). Biotech Week. September 17, 2025; p 833.