Bioinformatics Analysis Reveals Aging Hallmark Gene Set and Potential Biomarkers for Coronary Artery Disease

Strategic Insights -

Research conducted at Guangxi Medical University in Guangxi, People's Republic of China, has led to the identification of a set of genes associated with aging and their potential role in coronary artery disease (CAD). The study aimed to investigate how aging hallmarks exert their roles in CAD and identified differentially expressed genes (DEGs) and microRNAs (DEMis) in CAD microarray datasets. The researchers constructed an miR-mRNA network using Cytoscape and assessed the diagnostic utility of DEMis in CAD using receiver operating characteristic curve analysis.

Key Takeaways:

  • The study identified an aging hallmark gene set, comprising 18 genes, with TP53 as the central hub protein-protein interaction and microRNA-mRNA networks.
  • Two DEMis, hsa-miR-423-5p and hsa-miR-564, were found to potentially regulate TP53, rendering them potential CAD biomarkers.
  • Hsa-miR-564 emerged as a potential protective factor against CAD, with an area under the receiver operating characteristics curve (AUC) of 0.7261 in validation experiments.
  • Combining hsa-miR-423-5p, hsa-miR-564, total cholesterol (TC), high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), and white blood cells (WBC) achieved an AUC of 0.783.
  • The study suggests that hsamiR-564 could be a potential therapeutic target for CAD prevention or treatment.

Statistics:

  • 18 genes were identified as part of the aging hallmark gene set.
  • 2 DEMis, hsa-miR-423-5p and hsa-miR-564, were discovered to potentially regulate TP53.
  • The AUC for hsa-miR-564 in validation experiments was 0.7261.
  • The AUC for the combination of hsa-miR-423-5p, hsa-miR-564, TC, HDL-C, LDL-C, and WBC was 0.783.

Sources:

  • Bioinformatic analyses and validated experiments reveal an aging hallmark gene set and protective miR of coronary artery disease. Scientific Reports, 2025;15(1):32102.
  • Nature Portfolio, Heidelberger Platz 3, Berlin, 14197, Germany. (Nature Publishing Group - www.nature.com/; Scientific Reports - www.nature.com/srep/)
  • Guangxi Medical University, Dept. of Pathophysiology, School of Pre-clinical Medicine, Guangxi, People's Republic of China.