Breakthrough in Human Papillomavirus Detection using Electrochemical Sensor

Strategic Insights -

Researchers at Sogang University have developed a novel electrochemical sensor on a thermally controllable paper-based digital microfluidic (e-pDMF) device for detecting human papillomavirus type 16 DNA (HPV16 DNA). The e-pDMF device operates in transport and thermal modes to enable programmable droplet manipulation and precise temperature control, facilitating multiple processes from sample loading and mixing to signal amplification and electrochemical detection within 75 minutes.

Key Takeaways:

  • The e-pDMF device demonstrated a detection range from 1 fM to 10 pM with a high sensitivity and specificity for HPV16 DNA.
  • The platform showed no cross-reactivity to mismatched sequences or related genital tract pathogens, indicating its potential as an alternative point-of-care (POCT) tool for DNA diagnosis.
  • The research was conducted by a team of researchers from Sogang University, led by Sarath Kin, and published in the journal Biosensors and Bioelectronics.
  • The device maintained a precise isothermal state of 37.5 ± 0.14 °C and performed efficient Exonuclease III activity during signal amplification.
  • The platform facilitated multiple processes, reducing the total assay time to 75 minutes, and demonstrated a detection limit of 1 fM.

Statistics:

  • The e-pDMF device detected HPV16 DNA in the range of 1 fM to 10 pM with a correlation coefficient of 0.9968.
  • The platform demonstrated 100% agreement with PCR results when validated using clinical cervical swab samples.
  • The device operated with a thermal ramp rate of 0.09 °C/s and maintained a precise temperature control.
  • The total assay time was reduced to 75 minutes using the e-pDMF device.

Sources:

  • High-sensitivity electrochemical detection of HPV DNA via enzyme-amplified target-induced hairpin opening on a thermally controlled paper-based digital microfluidic platform. Biosensors and Bioelectronics, 2025;289:117898.
  • Biosensors and Bioelectronics can be contacted at: Elsevier Advanced Technology, Oxford Fulfillment Centre The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biosensors and Bioelectronics - www.journals.elsevier.com/biosensors-and-bioelectronics/)
  • Contact information for the research team: Sogang University, Dept. of Chemistry and Institute of Biological Interfaces, Seoul, 04107, South Korea.