Breakthrough in Nanozymes: Enhancing Peroxidase-Like Activity

Strategic Insights -

Researchers from Nagoya University have made a significant discovery in the field of nanotechnology, specifically in the development of nanozymes. By creating a highly efficient mesoporous medium-entropy alloy (m-MEA) nanozyme, the team has successfully enhanced peroxidase-like activity, paving the way for improved immunoassay applications. This breakthrough has the potential to revolutionize the field of health and medicine, particularly in the detection of diseases.

Key Takeaways:

  • The researchers developed a mesoporous medium-entropy alloy (m-MEA) nanozyme that exhibits exceptional stability and catalytic activity in the activation of HO, making it an ideal substitute for peroxidase (POD)-like enzymes in immunoassay applications.
  • The m-MEA nanozyme displays a high POD specific activity of 304 ± 1.69 U mg, which is 6.58- and 507-folds higher than that of mesoporous Pt and nonporous MEA nanozymes, respectively.
  • The exposure of mesoporous structure of m-MEA nanozyme ensures accessible active sites, resulting in a high POD specific activity.
  • The detectable levels of prostate-specific antigens (PSA) were exceptionally low at 1.20 pg mL, surpassing the sensitivity of traditional enzyme-linked assays.
  • The m-MEA-based immunoassay platform has shown potential in detecting diseases with high sensitivity and specificity.
  • The research has been peer-reviewed and published in ACS Nano.

Statistics:

  • The m-MEA nanozyme exhibits a high POD specific activity of 304 ± 1.69 U mg.
  • The detectable levels of PSA were exceptionally low at 1.20 pg mL.
  • The sensitivity of the m-MEA-based immunoassay platform is 15.34-fold higher than traditional enzyme-linked assays.

Sources:

  • Modulating p-d Orbital Hybridization in Mesoporous Medium-Entropy Alloy Nanozymes with Enhanced Peroxidase-Like Activity. ACS Nano, 2025.
  • Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; ACS Nano - www.pubs.acs.org/journal/ancac3)
  • Yoshi Suzuki, et al. (2023), Department of Materials Process Engineering, Graduate School of Engineering, Nagoya University.