Complete Genome Sequence of Lactiplantibacillus Plantarum Strain GA_C_14 May Aid in Swine Industry

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Researchers from Dankook University in South Korea have made a groundbreaking discovery in the field of animal science and technology. The team, led by Sumin Ryu and including Hyunok Doo and Eun Sol Kim, has successfully sequenced and analyzed the complete genome of Lactiplantibacillus plantarum strain GA_C_14. The genome, consisting of one circular chromosome and one circular plasmid, contains 3,083 protein-coding sequences, 67 tRNAs, and 16 rRNAs. Notably, the strain possesses enzymes involved in vitamin B6 metabolism and beta-galactosidase, making it a potentially functional probiotic for the swine industry.

Key Takeaways:

  • The complete genome sequence of Lactiplantibacillus plantarum strain GA_C_14 was sequenced and analyzed by researchers at Dankook University.
  • The genome consists of one circular chromosome (3,196,348bp) and one circular plasmid (40,211bp) with specific GC contents.
  • The strain harbors enzymes involved in vitamin B6 metabolism, including gapB (EC 1.2.1.12), SerC (EC 2.6.1.52), dxs (EC 2.2.1.7), SerA (EC 1.1.1.95), PdxK (EC 2.7.1.35), and PdxH (EC 1.4.3.5).
  • The strain also possesses the beta-galactosidase (EC 3.2.1.23) enzyme required for synthesizing galactooligosaccharides (GOS).
  • The research suggests that L. plantarum strain GA_C_14 could be utilized as a functional probiotic in the swine industry.
  • The study was published in the Journal of Animal Science and Technology (67(4):944-947) and is available online through a DOI link.
  • The research was funded by the Ministry of Food and Drug Safety.

Statistics:

  • The genome of L. plantarum strain GA_C_14 consisted of 1 circular chromosome (3,196,348bp) and 1 circular plasmid (40,211bp).
  • The GC content of the chromosomal DNA was 44.7%.
  • The GC content of the plasmid DNA was 38.9%.
  • The genome annotation revealed 3,083 protein-coding sequences (CDSs), 67 tRNAs, and 16 rRNAs.
  • The L. plantarum strain GA_C_14 possesses enzymes involved in vitamin B6 metabolism and beta-galactosidase.

Sources:

  • Ryu, S., Doo, H., Kim, E. S., Keum, G. B., Kwak, J., Pandey, S., ... & Lee, J. H. (2023). Complete genome sequence of Lactiplantibacillus plantarum strain GA_C_14 with potential characteristics applicable in the swine industry. Journal of Animal Science and Technology, 67(4), 944-947. doi: 10.5187/jast.2023.e134
  • NewsRx. Dankook University Researchers Target Animal Science and Technology (Complete genome sequence of Lactiplantibacillus plantarum strain GA_C_14 with potential characteristics applicable in the swine industry). Science Letter. September 5, 2025; p 57.