Effective Gene Editing System for Fraxinus mandshurica Developed

Strategic Insights -

Researchers at Shenyang Agricultural University have developed an effective CRISPR/Cas9 gene editing system for Fraxinus mandshurica, allowing for the improvement of its traits through genetic engineering methods. The system is based on the optimization of Agrobacterium tumefaciens concentration and infection duration, and a tissue culture system for clustered buds was developed by supplementing the media with hormones at different concentrations. The study successfully generated FmbHLH1-edited chimeric plants, induced and screened homozygous plants, and found that 18% of the induced clustered buds were gene-edited. The research concluded that the established CRISPR/Cas9 gene editing system was effective in regulating drought tolerance and adjusting the ability to scavenge reactive oxygen species and regulate osmotic potential.

Key Takeaways:

  • Researchers at Shenyang Agricultural University developed an effective CRISPR/Cas9 gene editing system for Fraxinus mandshurica using a growth points transformation method.
  • The system optimized Agrobacterium tumefaciens concentration and infection duration, and a tissue culture system for clustered buds was developed using hormone supplements.
  • FmbHLH1-edited chimeric plants were successfully generated, and 18% of the induced clustered buds were gene-edited, confirming the effectiveness of the system.
  • Homozygous plants were induced and screened using the developed clustered bud system, demonstrating the system's ability to regulate drought tolerance and adjust reactive oxygen species scavenging and osmotic potential.
  • The study improved the understanding of the genetic basis of drought tolerance in Fraxinus mandshurica and provided a method for the molecular breeding of the species.
  • Lei Li, Yucheng Wang, Fan Yang, Weiqi Li, Qiang Wang, Xuefei Xu, Yueliang Wu, Xiaoyu Ji, and other researchers contributed to this study, which highlights the potential of gene editing for improving crop traits.
  • The research was funded by The National Key R&D Program of China and published in the BMC Plant Biology journal.

Statistics:

  • 100 randomly transformed growing points were used to evaluate the efficacy of the CRISPR/Cas9 gene editing system.
  • 18% of the induced clustered buds were gene-edited, confirming the effectiveness of the system.
  • The study found that the developed CRISPR/Cas9 gene editing system was effective in regulating drought tolerance.
  • The system adjusted the ability to scavenge reactive oxygen species and regulate osmotic potential.

Sources:

  • "Establishment of a CRISPR/Cas9 gene editing system based on growth points transformation method in Fraxinus mandshurica." BMC Plant Biology, 2025,25(1):1-12.
  • https://doi-org.sdpl.idm.oclc.org/10.1186/s12870-025-07094-5
  • NewsRx. Studies from Shenyang Agricultural University Have Provided New Data on Gene Editing (Establishment of a CRISPR/Cas9 gene editing system based on growth points transformation method in Fraxinus mandshurica). Biotech Week. September 17, 2025; p 1099.