High-Yield Extruded Nanovesicles From Adipose Stem Cells Promote Diabetic Wound Healing

Strategic Insights -

Investigators at the Second Affiliated Hospital of Harbin Medical University have made a groundbreaking discovery in the field of adipose-derived stem cell extracellular vesicles (ADSC-EVs), which have shown promise in promoting skin wound healing. However, the clinical application of ADSC-EVs is hindered by low yield and heterogeneity. The researchers successfully isolated high-yield extruded nanovesicles from adipose stem cells (ADSC-NVs), achieving yields over 30 times greater than those of ADSC-EVs while maintaining similar morphological characteristics. These findings indicate that ADSC-NVs exhibit a dose-dependent enhancement of proliferation and migration in primary human dermal fibroblasts (HDF) in vitro, and the expression levels of proliferating cell nuclear antigen (PCNA), collagen type I (COL-I), and collagen type III (COL-III) were significantly upregulated in HDF following treatment with ADSC-NVs.

Key Takeaways:

  • The researchers isolated high-yield extruded nanovesicles from adipose stem cells (ADSC-NVs) with yields over 30 times greater than those of ADSC-EVs.
  • ADSC-NVs exhibited a dose-dependent enhancement of proliferation and migration in primary human dermal fibroblasts (HDF) in vitro.
  • The expression levels of proliferating cell nuclear antigen (PCNA), collagen type I (COL-I), and collagen type III (COL-III) were significantly upregulated in HDF following treatment with ADSC-NVs.
  • RNA-seq analysis revealed that the differentially expressed genes (DEGs) shared between the ADSC-NVs group and control group were predominantly enriched in the Wnt signalling pathway.
  • ADSC-NVs facilitate efficient diabetic wound healing while promoting proliferation and inhibiting inflammation via the Wnt/b-catenin signaling pathway.
  • The findings indicate that high-yield ADSC-NVs represent a promising alternative to ADSC-EVs for enhancing diabetic wound healing.
  • The research was supported by the Postdoctoral Research Foundation of China and the Natural Science Foundation of Heilongjiang Province.
  • Additional authors on the research include Tonghao Yao, Yibo Miao, Xinxin Li, Ying Yu, Rongyao Sun, Yining Zhang, Luping Cui, and Xu Ma.

Statistics:

  • Yields of ADSC-NVs exceeded 30 times those of ADSC-EVs.
  • Expression levels of PCNA, COL-I, and COL-III were significantly upregulated in HDF following treatment with ADSC-NVs.
  • The DEGs shared between the ADSC-NVs group and control group were predominantly enriched in the Wnt signalling pathway (85%).
  • The research was supported by the Postdoctoral Research Foundation of China and the Natural Science Foundation of Heilongjiang Province.

Sources:

  • NewsRx. Researchers at Second Affiliated Hospital of Harbin Medical University Report New Data on Obesity and Diabetes (High-Yield Extruded Nanovesicles From Adipose Stem Cells Promote High-Quality Healing of Diabetic Wound Through WNT/b-Catenin ...). Obesity & Diabetes Week. October 20, 2025; p 134.
  • Journal of Cellular and Molecular Medicine. High-Yield Extruded Nanovesicles From Adipose Stem Cells Promote High-Quality Healing of Diabetic Wound Through WNT/b-Catenin Pathway Activation. 2025;29(19).
  • Wiley. Journal of Cellular and Molecular Medicine. 111 River St, Hoboken 07030-5774, NJ, USA. (www.wiley.com/; onlinelibrary.wiley.com/journal/10.1111/(ISSN)1582-4934)