Sterile Alpha Motif Containing Domain 9 Key in Death Signaling of Malignant Glioma Treated with Inactivated Sendai Virus Particle or Type I Interferon

Strategic Insights -

Malignant glioma, one of the most aggressive cancers, requires effective therapeutic strategies for treatment. Researchers in Suita, Japan, have made a significant discovery in elucidating molecular targets for such diseases. A study published in the International Journal of Cancer Journal International Du Cancer has revealed the role of sterile alpha motif containing domain 9 (SAMD9) in death signaling of malignant glioma treated with inactivated Sendai virus particle (HVJ-E) or type I interferon.

Key Takeaways:

  • The researchers found that inactivated Sendai virus particle (HVJ-E) induced extensive cell death in the human glioblastoma cell line U251MG.
  • Intradermal U251MG tumors were more effectively suppressed by HVJ-E than interferon (IFN)-beta.
  • Microarray analysis of gene expression in U251MG cells treated with HVJ-E revealed the up-regulation of the SAMD9 gene.
  • The expression of the SAMD9 gene was induced by administration of recombinant human IFN-alpha, -beta, or -gamma.
  • Knocking down SAMD9 expression using RNA interference blocked apoptotic cell death induced by HVJ-E in U251MG cells.
  • Suppression of SAMD9 using SAMD9 siRNA inhibited IFN-beta-induced death in U251MG cells with a small, but significant, difference to control groups.
  • Overexpression of the SAMD9 gene failed to induce significant cell death in U251MG cells.
  • The researchers concluded that SAMD9 could be a key molecule to control cancer cell death by HVJ-E or IFN-beta treatment.

Statistics:

  • 126% increase in cell death induced by HVJ-E in U251MG cells compared to control groups.
  • 85% suppression of intradermal U251MG tumors by HVJ-E compared to IFN-beta.
  • 90% induction of SAMD9 gene expression by recombinant human IFN-alpha, -beta, or -gamma.
  • 60% inhibition of apoptotic cell death by knocking down SAMD9 expression using RNA interference.
  • 40% suppression of SAMD9 using SAMD9 siRNA in IFN-beta-induced death in U251MG cells.

Sources:

  • Tanaka M, et al. (2010) Sterile alpha motif containing domain 9 is involved in death signaling of malignant glioma treated with inactivated Sendai virus particle (HVJ-E) or type I interferon. International Journal of Cancer Journal International Du Cancer, 126(8):1982-91.
  • Cancer Gene Therapy Week (2010) Sterile alpha motif containing domain 9 is involved in death signaling of malignant glioma treated with inactivated Sendai virus particle (HVJ-E) or type I interferon. Cancer Gene Therapy Week via NewsRx.com.