Biochemistry
Novel Primer-Less Amplification Method for Detection of RNA Molecules
Researchers at the Centro de Biologia Molecular Severo Ochoa in Madrid, Spain, have developed a novel primer-less amplification method for the direct detection of SARS-CoV2 RNA. This method, called Hyperbranched Rolling Circle Amplification (HRCA), uses three enzymes: SplintR, TthPrimPol, and Qx5, a thermostabilized variant of phi29 DNA polymerase. The HRCA