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Enzyme-linked immunosorbent assay

Development and Validation of a Double-Antibody Sandwich ELISA for Detection of Anguillid Herpesvirus

Strategic Insights

— 1 min read

Researchers from the Fujian Academy of Agricultural Sciences in Fuzhou, People's Republic of China, have developed a new diagnostic tool for detecting Anguillid Herpesvirus (AngHV), a highly pathogenic agent causing 'Mucus sloughing and hemorrhagic septicemia disease' in eels. The double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was established for the detection of AngHV, employing monoclonal antibodies (mAbs) and polyclonal antibodies. The study demonstrated the effectiveness of the assay in detecting AngHV in various tissues of infected eels, with a detection limit of 10,000 copies and an 88.89% concordance rate with quantitative real-time PCR (qPCR).

Key Takeaways:

  • The Anguillid Herpesvirus (AngHV) is a highly pathogenic agent that causes 'Mucus sloughing and hemorrhagic septicemia disease' in eels, resulting in high morbidity and cumulative mortality rates.
  • Three monoclonal antibodies (mAbs) against AngHV were developed and validated, showing high specificity and affinity.
  • A double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was established for the detection of AngHV, employing mAb 10G8-9F4 as the capture antibody and rabbit polyclonal antibody 9NA as the detection antibody.
  • The developed assay effectively identified AngHV in various tissues of infected eels, including heart, liver, spleen, kidney, gills, intestines, muscle, skin mucus, and fins.
  • The DAS-ELISA demonstrated high reactivity and specificity, serving as a rapid and effective diagnostic tool for the detection and prevention of AngHV in eel populations.

Statistics:

  • The detection limit of the DAS-ELISA was determined to be 10,000 copies of AngHV.
  • The assay had an 88.89% concordance rate with quantitative real-time PCR (qPCR) in the analysis of clinical samples.
  • The study found no cross-reactivity with other common aquatic viruses, including Koi herpesvirus (KHV), Grass carp reovirus (GCRV), Infectious pancreatic necrosis virus (IPNV), and Large yellow croaker iridovirus (LYCI).

Sources:

  • Development and Validation of a Double-Antibody Sandwich ELISA (DAS-ELISA) for Detection of Anguillid Herpesvirus. Journal of Fish Diseases, 2025.
  • NewsRx. Findings from Fujian Academy of Agricultural Sciences in the Area of Immunoglobulins Described [Development and Validation of a Double-Antibody Sandwich ELISA (DAS-ELISA) for Detection of Anguillid Herpesvirus]. Life Science Weekly. November 4, 2025; p 921.

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