Alfalfa Sprouts and Seeds Valorization in Enriched Sourdough Bread

Researchers from the University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Romania, have investigated the potential of using alfalfa sprout flour and alfalfa seed flour in wheat-based bread formulations with sourdough fermentation. Their study found that the addition of alfalfa flour enhanced the bread's content in protein, total free amino acids, dietary fiber, minerals, and polyphenols. The research also highlighted the importance of microbial fermentation in developing functional bakery products with enhanced nutritional and sensory qualities.

Key Takeaways:

  • The study investigated the potential of using alfalfa sprout flour (AGF) and alfalfa seed flour (ASF) in wheat-based bread formulations with sourdough fermentation.
  • The results indicated that both AGF and ASF enhanced the bread's content in protein by 1.03 to 1.27 times, total free amino acid by 1.08 to 2.1 times, dietary fiber by 1.12 to 1.85 times, minerals, and polyphenols by 1.12 to 2.36 times.
  • Bread samples with moderate AGF inclusion (5 g) fermented with S. cerevisiae were most appreciated by the sensory panel, suggesting a balance between nutritional enrichment and desirable organoleptic properties.
  • The research highlighted the potential of alfalfa and microbial fermentation in developing functional bakery products with enhanced nutritional and sensory qualities.
  • The study was conducted by Delia-Elena Pausan, Adriana Paucean, Simona Maria Man, Maria Simona Chis, Carmen-Rodica Pop, Anamaria Pop, and Floricuta Ranga from the University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Romania.
  • The research was published in the Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology, 2025, 82(2).

Statistics:

  • The addition of alfalfa flour enhanced the bread's content in protein by 1.03 to 1.27 times.
  • The addition of alfalfa flour enhanced the bread's content in total free amino acid by 1.08 to 2.1 times.
  • The addition of alfalfa flour enhanced the bread's content in dietary fiber by 1.12 to 1.85 times.
  • The addition of alfalfa flour enhanced the bread's content in minerals and polyphenols by 1.12 to 2.36 times.
  • Twelve experimental bread samples were prepared with varying levels of AGF (0-15 g) and constant ASF content (5 g).

Sources:

  • Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology, 2025, 82(2).
  • Delia-Elena Pausan, Adriana Paucean, Simona Maria Man, Maria Simona Chis, Carmen-Rodica Pop, Anamaria Pop, and Floricuta Ranga. Alfalfa Sprouts and Seeds Valorization in Enriched Sourdough Bread. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology, 2025, 82(2).
  • University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca. Researchers Report Recent Findings in Agricultural Science (Alfalfa Sprouts and Seeds Valorization in Enriched Sourdough Bread). Pharma Business Week. October 20, 2025; p 144.