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Forestry

Genome-Wide Identification of Calcineurin B-like (Cbl) and Cbl-Interacting Protein Kinase (Cipk) Gene Families in Nicotiana Benthamiana

Strategic Insights

— 1 min read

Scientists at Zhejiang Agriculture and Forestry University have made significant discoveries regarding the Calcineurin B-like (CBL) and CBL-interacting Protein Kinase (CIPK) gene families in Nicotiana benthamiana, a crucial model organism used in gene study and molecular farming. The research, which has been peer-reviewed, identifies 17 CBLs and 48 CIPKs in N. benthamiana, and provides novel insights into the functional exploration of the CBL-CIPK molecule, as well as a valuable resource for antiviral genetic enhancement.

Key Takeaways:

  • The research identifies 17 CBLs and 48 CIPKs in N. benthamiana, with 4 and 7 distinct clades, respectively, each exhibiting conserved motif and gene structures.
  • Segmental duplications were identified as a major driver for duplication patterns, and both NbCBLs and NbCIPKs were unevenly distributed across 19 chromosomes, with a relatively weak codon usage bias.
  • The expression levels of NbCBLs and NbCIPKs varied across distinct tissues, and were influenced by viral infection.
  • Silencing of NbCBL10 increased viral susceptibility.
  • The research offers a valuable resource for antiviral genetic enhancement.

Statistics:

  • 17 CBLs and 48 CIPKs were identified in N. benthamiana.
  • 4 distinct clades of CBLs and 7 distinct clades of CIPKs were identified.
  • 19 chromosomes were unevenly distributed across NbCBLs and NbCIPKs.
  • The codon usage bias was relatively weak.

Sources:

  • NewsRx. Findings from Zhejiang Agriculture and Forestry University Yields New Data on Calcineurin [Genome-wide Identification, Evolution and Expression Analysis of the Calcineurin B-like (Cbl) and Cbl-interacting Protein Kinase (Cipk) Gene Families ...]. Biotech Business Week. October 20, 2025; p 34.
  • Chen, J., He, L., Yao, K., Cui, S., Cao, H., and Zhang, S. Genome-wide Identification, Evolution and Expression Analysis of the Calcineurin B-like (Cbl) and Cbl-interacting Protein Kinase (Cipk) Gene Families Under Viral Stress In Nicotiana Benthamiana. Industrial Crops and Products, 2025;234.

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