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Genetic research

Investigating the Genetic Diversity of Bipolaris oryzae Isolates in Rice Cultivation

Strategic Insights

— 1 min read

Researchers from Sunchon National University have conducted a study to understand the population structure and genetic diversity of Bipolaris oryzae isolates, the causative agent of rice brown spot disease. The disease typically occurs during the rice harvest season and can lead to significant yield losses. The study aimed to investigate the genetic diversity of 50 B. oryzae isolates collected from rice infected with brown spot through random amplified polymorphic DNA analysis.

Key Takeaways:

  • The study found that among 140 primers tested, 30 were selected and applied, of which 5 exhibited significant polymorphisms among the isolates.
  • The generated dendrogram revealed five clades with 92% similarity, with Group A being the most predominant, comprising 84.0% of the total isolates (42/50).
  • The study selected 10 strains from different groups and tested them for their mycological characteristics and pathogenicity, with F1253, F1317, and F1409 exhibiting higher conidial production and causing larger diseased leaf areas.
  • The results suggest that the B. oryzae isolates that caused rice brown spot in 2023 are genetically homogeneous.
  • The study may serve as a basis for developing targeted control strategies against brown spot.

Statistics:

  • 50 B. oryzae isolates were collected from rice infected with brown spot.
  • 140 primers were tested, with 30 being selected and applied.
  • 5 primers exhibited significant polymorphisms among the isolates.
  • The generated dendrogram revealed five clades with 92% similarity.
  • Group A comprised 84.0% of the total isolates (42/50).
  • 2 strains from Group A, 1 each from Groups B, C, D, and E were selected and tested for their mycological characteristics and pathogenicity.
  • F1253, F1317, and F1409 exhibited higher conidial production and caused larger diseased leaf areas in the pathogenicity tests.

Sources:

  • "Random Amplified Polymorphic DNA-Based Analysis of Genetic Diversity and Characterization of Representative Bipolaris oryzae Isolates from 2023-2024. The Plant Pathology Journal, 2025,41(5):671-681"
  • The Plant Pathology Journal, https://www.ppjonline.org/
  • Sunchon National University, Suncheon, South Korea
  • Seoyeon Kim, Department of Plant Medicine, Sunchon National University
  • Hyunjung Chung, Seol-Hwa Jang, Shinhwa Kim, Ki-Tae Kim, Sook-Young Park, authors of the study

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