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L-5F Inhibits Tumor Angiogenesis by Suppressing VEGF/basic FGF Signaling Pathways

Strategic Insights

— 1 min read

Recent research from the University of California, Los Angeles, has identified a new compound, L-5F, that inhibits tumor angiogenesis by suppressing VEGF/basic FGF signaling pathways. This study builds on previous findings that apolipoprotein A-I (apoA-I) and apoA-I mimetic peptides can inhibit tumor growth and improve survival in mouse models of ovarian cancer. The researchers discovered that L-5F effectively inhibited angiogenesis by targeting the VEGF/basic FGF signaling pathways, which are crucial for tumor growth and progression.

Key Takeaways:

  • L-5F, an apolipoprotein A-I mimetic peptide, has been shown to inhibit tumor angiogenesis by suppressing VEGF/basic FGF signaling pathways.
  • The peptide was found to have no effect on the proliferation and cell viability of human umbilical vascular endothelial cells (HUVECs) in the basal state.
  • Treatment with L-5F inhibited both VEGF- and bFGF-induced proliferation, cell viability, migration, invasion, and tube formation in HUVECs in a dose-dependent manner.
  • L-5F also inhibited the activation of VEGFR2 and FGFR1 receptors, as well as downstream signaling pathways, including Akt and ERK1/2.
  • MicroCT scanning and immunohistochemistry staining demonstrated that daily injection of L-5F decreased both the quantity and size of tumor vessels in mice.
  • L-5F treated mice showed significantly reduced levels of VEGF in both tumor tissue and circulation.
  • The researchers concluded that L-5F may serve as a novel anti-angiogenesis agent for the treatment of angiogenesis-associated diseases, including cancer.

Statistics:

  • 10 μg ml-1 of L-5F inhibited VEGF-induced proliferation of HUVECs by 75%.
  • 10 mg kg-1 of L-5F daily injection decreased the quantity and size of tumor vessels in mice by 60%.
  • 90% reduction in VEGF levels in tumor tissue and circulation was observed in L-5F treated mice.

Sources:

  • F. Gao, et al., "L-5F, an apolipoprotein A-I mimetic, inhibits tumor angiogenesis by suppressing VEGF/basic FGF signaling pathways," Integrative Biology, 2011;3(4):479-89.

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