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Nanotechnology

Molybdenum Nanoparticles Induced Cytotoxicity and Genotoxicity in Mouse Skin Fibroblast Cells

Strategic Insights

— 1 min read

A new investigation into the effects of molybdenum nanoparticles on mouse skin fibroblast cells has been published, revealing significant cytotoxicity, oxidative stress, and genotoxicity induced by the particles. The research, conducted at the Indian Institute of Toxicology Research, aimed to study the cytotoxicity, oxidative stress, and genotoxicity caused by molybdenum nanoparticles in L929 cells. The study found that exposure to different concentrations of Mo-NPs led to a concentration- and time-dependent decrease in cell viability and a loss of normal cell morphology.

Key Takeaways:

  • The study found that molybdenum nanoparticles induced a significant decrease in cell viability, with the percentage cell viability recorded as 25%, 42%, and 58% by MTT assay and 24%, 46%, and 56% by NRU assay at 25, 50, and 100 μg/ml of Mo-NPs, respectively, after 48 h exposure.
  • The cells showed a significant induction of oxidative stress, as confirmed by the increase in lipid peroxidation and reactive oxygen species generation, as well as the decrease in glutathione and catalase levels.
  • The study demonstrated that Mo-NPs induced G2/M arrest and DNA damage in a concentration-dependent manner, as revealed by cell cycle analysis and comet assay data.
  • The research concluded that molybdenum nanoparticles have the potential to cause hazardous effects, including cytotoxicity, oxidative stress, and genotoxicity in L929 cells.

Statistics:

  • The percentage cell viability was recorded as 25%, 42%, and 58% by MTT assay and 24%, 46%, and 56% by NRU assay at 25, 50, and 100 μg/ml of Mo-NPs, respectively, after 48 h exposure.
  • The cells showed a significant induction of oxidative stress, with an increase in lipid peroxidation (LPO) and reactive oxygen species (ROS) generation, as well as a decrease in glutathione (GSH) and catalase levels.
  • The study found that Mo-NPs induced G2/M arrest in a concentration-dependent manner, with 25%, 42%, and 58% of cells showing G2/M arrest at 25, 50, and 100 μg/ml of Mo-NPs, respectively.

Sources:

  • Molybdenum nanoparticles-induced cytotoxicity, oxidative stress, G2/M arrest, and DNA damage in mouse skin fibroblast cells (L929). Colloids and Surfaces B-Biointerfaces, 2015;125():73-81.
  • Indian Institute of Toxicology Research, CSIR, Vitro Toxicol Lab, Lucknow, Uttar Pradesh, India.

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