Pediococcus Acidilactici Strain P10: A Promising Probiotic for Animal Health

Research conducted at the National Institute of Genetic Engineering and Biotechnology in Tehran, Iran, has identified a novel probiotic strain, Pediococcus acidilactici P10, isolated from native broiler chickens. This strain demonstrates promising probiotic attributes, including non-hemolytic properties, high survival rates in simulated gastrointestinal conditions, and potent antimicrobial activity against key pathogens. The whole-genome sequencing of P10 has revealed a complete, functional Type II-A CRISPR-Cas system, which represents a robust antiviral defense mechanism, and 59 genes unique to P10 not found in other P. acidilactici strains.

Key Takeaways:

• The study presents a comprehensive characterization of Pediococcus acidilactici strain P10, a novel probiotic isolated from native broiler chickens.
• P10 demonstrates non-hemolytic properties and high survival rates under simulated gastrointestinal conditions (95-99% in acidic pH, 55% in bile salts).
• The strain exhibits potent broad-spectrum antimicrobial activity against key pathogens, with genomic analysis confirming the presence of associated stress resistance genes.
• P10 displays strong adherence to intestinal epithelial cells, with corresponding adhesion genes identified in its genome.
• The whole-genome sequencing of P10 revealed a complete, functional Type II-A CRISPR-Cas system and 59 genes unique to P10 not found in other P. acidilactici strains.
• The research concludes that Pediococcus acidilactici strain P10 is a highly promising probiotic for applications in animal health and functional foods.

Statistics:

• 95-99% survival rate of P10 in acidic pH
• 55% survival rate of P10 in bile salts
• 17 phage-matching spacers in P10's CRISPR-Cas system
• 59 genes unique to P10 not found in other P. acidilactici strains

Sources:

• Comprehensive in vitro and whole-genome characterization of probiotic properties in Pediococcus acidilactici P10 isolated from Iranian broiler chicken (Scientific Reports, 2025;15(1):28953)
• National Institute of Genetic Engineering and Biotechnology (Tehran, 8415683111, Iran)
• National Institute of Genetic Engineering and Biotechnology: Author information (Sahar Mahmoodian, Raana Tabashiri, Mohammad Hossein Pakdel, Vahid Shariati, Amir Meimandipour, Javad Zamani)
• Nature Portfolio (Heidelberger Platz 3, Berlin, 14197, Germany)
• Nature Publishing Group (www.nature.com/)
• Scientific Reports (www.nature.com/srep/)
• Life Science Weekly (August 26, 2025; p 4992)