Researchers at Guangdong Ocean University Report Research in Pore Forming Cytotoxic Proteins

Researchers at Guangdong Ocean University have detailed new findings in the field of pore forming cytotoxic proteins. The study, which investigated the effects of bacteriocin produced by Bacillus velezensis G02 on the intestinal microbiota of normal mice, revealed significant changes in the diversity and evenness of the intestinal microbiota. The study also identified a putative novel bacteriocin, bacteriocin PG02, which exhibited broad-spectrum antibacterial activity against both gram-positive and gram-negative bacteria. The research provides a reference for the microbiology field research and development of feed additives.

Key Takeaways:

• The study focused on the effects of bacteriocin produced by Bacillus velezensis G02 on the intestinal microbiota of normal mice, revealing significant changes in the diversity and evenness of the intestinal microbiota.
• The study identified a putative novel bacteriocin, bacteriocin PG02, which exhibited broad-spectrum antibacterial activity against both gram-positive and gram-negative bacteria.
• The molecular weight of the purified bacteriocin was found to be 20.1 ~ 31 kDa, and it was speculated to be a putative novel bacteriocin.
• The study concluded that the bacteriocin crude extract had no obvious adverse reactions on the internal organs of mice, and no mice died.
• The study provided a reference for the microbiology field research and development of feed additives.

Statistics:

• The study used 16 S rDNA amplicon sequencing analysis to reveal that the crude extract of the bacteriocin significantly changed the a-diversity (Shannon (P = 0.51), Simpson (P = 0.51), and Chao1 (P = 0.28)) and b-diversity (PCoA, (P = 0.1) NMDS, (P = 0.0132)) of the intestinal microbiota in normal mice.
• The study found that the bacteriocin crude extract reduced the species abundance and increased the evenness of the intestinal microbiota in normal mice.
• The study used LC-MS/MS in combination with sequence coverage analysis to identify the purified bacteriocin, with a molecular weight of 20.1 ~ 31 kDa.

Sources:

• Identification and characterization of a putative novel bacteriocin from Bacillus velezensis G02 and its effects on the intestinal microflora in mice. BMC Microbiology, 2025, 25(1):1-16. (BMC Microbiology - http://www.biomedcentral.com/bmcmicrobiol/)
• Guangdong Ocean University, College of Coastal Agricultural Sciences, Guangdong Ocean University
• Mengbo Yu, Haotian Ma, Jinju Peng, Yuexia Ding, Qianqian Lin, Fucheng Guo, Yi Ma, Natural Science Foundation of Guangdong Province.