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Genetic research

Researchers Detail Findings in Genetics of G6 Transgenic Mutiara Catfish

Strategic Insights

— 2 min read

Investigators at the Universitas Padjadjaran have been studying the genetics of G6 transgenic mutiara catfish. Their research focused on the impact of stocking density on the expression of key genes involved in stress and immunity response. The study involved two-month-old TG and NTG fish, which were treated with different densities for 60 days. The results showed that TG fish had higher expression of certain genes, such as CgGH, THR, HSP70, TLR1, and IgM, particularly in high-density conditions. The study also found that NTG fish were more easily stressed, with higher white blood cell counts and cortisol levels compared to TG fish.

Key Takeaways:

  • The researchers used a stocking density difference test (30, 60, and 90 fish/400 L) and gene expression analysis of Clarias gariepinus growth hormone (CgGH), thyroid hormone receptor (THR), heat shock protein 70 (HSP70), toll-like receptor-1 (TLR1), and immunoglobulin M (IgM) to assess the immunity of G6 transgenic mutiara catfish.
  • The study found that TG fish had higher expression of CgGH, THR, HSP70, TLR1, and IgM genes in high-density conditions compared to low-density conditions and NTG fish.
  • The CgGH gene was found to modulate the expression of THR and HSP70 genes and increase the expression of TLR1 and IgM.
  • NTG fish had higher white blood cell counts and cortisol levels than TG fish, indicating that they were more easily stressed.
  • The study suggested that GH-transgenesis may enhance fish immunity to stress in high stocking density.
  • The researchers concluded that further research is needed to determine the relationship between genes and hormones involved in stress and immunity induction and fish density in transgenic and non-transgenic fish.

Statistics:

  • 30, 60, and 90 fish/400 L were used as stocking densities in the study.
  • The CgGH, THR, HSP70, TLR1, and IgM genes were more highly expressed in TG fish with high density (90 fish/400 L) with means of 4.75 ± 0.78; 5.09 ± 0.92; 4.76 ± 0.79; 17.79 ± 0.67; and 15.22 ± 0.63, respectively.
  • The CgGH, THR, HSP70, TLR1, and IgM genes were less highly expressed in TG fish with low density (30 fish/400 L) with means of 2.12 ± 0.60; 1.68 ± 0.75; 3.55 ± 0.75; 6.92 ± 0.57; and 3.53 ± 0.92, respectively.
  • NTG fish had higher white blood cell counts (means 46.20 ± 1.00 x 103 cells/mm3) and cortisol levels (means 69 ± 1.94 ng/mL) than TG fish (means 36.70 ± 1.01 x 103 cells/mm3 and 38.65 ± 1.43 ng/mL).

Sources:

  • Gene and hormones involved in stress density and immunity response of G6 transgenic mutiara catfish (Clarias gariepinus). Discover Applied Sciences, 2025,7(8):1-23. (Published by Springer)
  • NewsRx. Universitas Padjadjaran Researchers Detail Findings in Genetics [Gene and hormones involved in stress density and immunity response of G6 transgenic mutiara catfish (Clarias gariepinus)]. Life Science Weekly. September 2, 2025; p 7464.

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