Researchers Discover Binding Protein for Squid Ink Polysaccharides Using Magnetic Nanoparticles

A new study on nanotechnology has led to the discovery of a binding protein for squid ink polysaccharides using magnetic nanoparticles. The research, conducted by a team of scientists from Guangdong Ocean University, aimed to understand the action mechanisms of squid ink polysaccharides (SIP) and their bio-functions. The study prepared SIP binding proteins from mouse liver using superparamagnetic nanometer beads and detected the Michaelis-Menten constant (K-m) from a Lineweaver-Burk double reciprocal plot to assess the effect of SIP on the activity of aldehyde oxidase (AOX).

Key Takeaways:

• The research team prepared SIP binding proteins from mouse liver using superparamagnetic nanometer beads to understand the action mechanisms of SIP bio-functions.
• The study detected the Michaelis-Menten constant (K-m) from a Lineweaver-Burk double reciprocal plot to assess the effect of SIP on the activity of aldehyde oxidase (AOX).
• The research showed that three proteins, AOX-3, regucalcin (RGN), and alpha 1-antitrypsin (A1AT3), were separated from mouse liver by magnetic nanoparticles conjugated with SIP.
• The contents of AOX-3 were found to be much more than RGN and A1AT3.
• The study concluded that squid ink polysaccharides (SIP) reduced the K-m value of aldehyde oxidase of mouse liver from 91.79 μmol L-1 to 43.70 μmol L-1.
• Huazhong Liu, Guangdong Ocean University, was the corresponding author of the study.
• Additional authors included Zhen Lin, Xiaohui Tan, Fangping Li, Yu Zhang, Ping Luo, and Xuan Lin.

Statistics:

• The K-m value of aldehyde oxidase of mouse liver was reduced from 91.79 μmol L-1 to 43.70 μmol L-1 by squid ink polysaccharides (SIP).
• Three proteins, AOX-3, regucalcin (RGN), and alpha 1-antitrypsin (A1AT3), were separated from mouse liver by magnetic nanoparticles conjugated with SIP.
• The contents of AOX-3 were much more than RGN and A1AT3.

Sources:

• NewsRx. Researchers' Work from Guangdong Ocean University Focuses on Nanoparticles (Identification of Aldehyde Oxidase 3 As a Binding Protein for Squid Ink Polysaccharides Using Magnetic Nanoparticles). Nanotechnology Weekly. March 1, 2021; p 5561.
• Identification of Aldehyde Oxidase 3 As a Binding Protein for Squid Ink Polysaccharides Using Magnetic Nanoparticles. RSC Advances, 2021;11(6):3596-3602.
• RSC Advances can be contacted at: Royal Soc Chemistry, Thomas Graham House, Science Park, Milton Rd, Cambridge CB4 0WF, Cambs, England.