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Cancer

Sensitive Analysis of Tyrosine Kinase Inhibitors in Plasma Using Polypropylene Fabric-Solid Phase Extraction Combined with Liquid Chromatography-Tandem Mass Spectrometry

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— 1 min read

Researchers at the Tianjin Medical University Cancer Institute and Hospital have developed a novel solid phase extraction (SPE) method using hydrophobic polypropylene fabric (PPF) for the accurate quantification of tyrosine kinase inhibitors (TKIs) in plasma. This method enables sensitive determination of eight clinically relevant TKIs in plasma, with high extraction recoveries, good reproducibility, low detection limits, and high intra-/inter-day recoveries and precision. The PPF-SPE device offers significant advantages in operational simplicity, cost-effectiveness, high throughput, and environmental sustainability compared to traditional SPE methods.

Key Takeaways:

  • The novel SPE method employs hydrophobic polypropylene fabric (PPF) as the sorbent, eliminating the need for sieve plates and simplifying column assembly.
  • The method enabled sensitive determination of eight clinically relevant TKIs (afatinib, alectinib, ceritinib, dasatinib, sunitinib, pazopanib, tamoxifen, and ibrutinib) in plasma with high extraction recoveries and good reproducibility.
  • Key parameters, including PPF amount, sample pH, and desorption conditions, were systematically optimized to achieve high extraction recoveries (83.1-96.4 %) and good reproducibility (RSDs 0.99).
  • The PPF-SPE device showed low detection limits (0.017-0.055 ng/mL) and high intra-/inter-day recoveries (85.1 %-102.6 %) and precision (RSDs 0.99).
  • The method integrated with liquid chromatography-tandem mass spectrometry (LC-MS/MS) enabled the sensitive detection of TKIs in plasma.
  • The PPF-SPE device has significant advantages in operational simplicity, cost-effectiveness, high throughput, and environmental sustainability compared to traditional SPE methods.

Statistics:

  • 8 clinically relevant TKIs were detected in plasma using the novel PPF-SPE method.
  • Extraction recoveries ranged from 83.1-96.4 %.
  • Reproducibility had an RSD of 0.99.
  • Detection limits ranged from 0.017-0.055 ng/mL.
  • Intra-day and inter-day recoveries ranged from 85.1-102.6 %.

Sources:

  • Sensitive analysis of tyrosine kinase inhibitors in plasma using polypropylene fabric-solid phase extraction combined with liquid chromatography-tandem mass spectrometry. Journal of Chromatography A, 2025;1760:466315.
  • Elsevier. Journal of Chromatography A. www.journals.elsevier.com/journal-of-chromatography-a/
  • Tianjin Medical University Cancer Institute and Hospital. Key Laboratory of Cancer Prevention and Therapy. www.tmu.edu.cn
  • Dong Wang, Dept. of Pharmacy, Tianjin Medical University Cancer Institute and Hospital.
  • Mengrong Li, Tingting Dai, Xiangyu Zhang and Di Chen.

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