Sign in Subscribe
Proteins

Ultrasonic Treatment Enhances Protein Hydrolysates Properties

Strategic Insights

— 1 min read

Scientists at Jiangsu University in China investigated the influence of dual-frequency ultrasonic treatment on protein hydrolysates from housefly larvae. The study revealed that in situ treatment with 20 ± 2/28 ± 2 kHz dual-frequency ultrasonic irradiation significantly improved enzymolysis reaction kinetics, thermodynamics, and antioxidant activity. The treatment increased the reaction rate constant by 82.67%, decreased the Michaelis constant by 13.54%, and enhanced the maximum reaction rate by 6.12%. Additionally, the study found that the thermodynamic parameters decreased significantly, indicating a decrease in activation energy, enthalpy, and entropy. However, no significant change in Gibbs free energy was observed.

Key Takeaways:

  • Researchers used dual-frequency ultrasonic treatment to enhance protein hydrolysates from housefly larvae.
  • The treatment increased the reaction rate constant by 82.67%, decreased the Michaelis constant by 13.54%, and enhanced the maximum reaction rate by 6.12%.
  • The thermodynamic parameters decreased significantly, indicating a decrease in activation energy, enthalpy, and entropy.
  • No significant change in Gibbs free energy was observed.
  • The treatment also enhanced the in vitro antioxidant activity, inhibited intracellular reactive oxygen species expression, and promoted HepG2 cell proliferation.
  • The results indicated that the in situ dual-frequency ultrasonic treatment had a positive effect on the properties of protein hydrolysates from housefly larvae.

Statistics:

  • The reaction rate constant increased by 82.67% due to the dual-frequency ultrasonic treatment.
  • The Michaelis constant decreased by 13.54% following the treatment.
  • The maximum reaction rate was enhanced by 6.12% after the treatment.
  • The thermodynamic parameters - activation energy, enthalpy, and entropy - decreased by 24.59%, 23.95%, and 18.86%, respectively.
  • The IC values of protein hydrolysate obtained with in situ dual-frequency ultrasonic treatment for DPPH, hydroxyl, and superoxide anion radicals decreased from 3.02, 4.11, and 5.25 mg/mL to 2.64, 3.47, and 4.68 mg/mL, respectively.

Sources:

  • Influence of in situ 20 ± 2/28 ± 2 kHz dual-frequency ultrasonication on enzymolysis kinetics, thermodynamics and antioxidant activity of housefly (Musca Domestica) larvae protein hydrolysate. Ultrasonics Sonochemistry, 2025;122:107599.

Read more